|
KMID : 1094719990040040281
|
|
Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 4 p.281 ~ p.286
|
|
|
Kinetic model for biotransformation of digitoxin in plant cell suspension culture ofDigitalis lanata
|
|
Choi Jeong-Woo
Kim Young-Kee
Park Hyun-Kyu
Lee Won-Hong
Kim Dong-Il
|
|
|
Abstract
|
|
|
|
Batch suspension cultures ofDigitalis lanata plant cell were performed to investigate the biotransformation of digitoxin.Digitalis lanata K3OHD plant cells were used to biotransform digitoxin into deacetyllanatoside C. A kinetic model was proposed to describe cell growth, substrate consumption, depletion of digitoxin, formation and depletion of digoxin and purpureaglycoside A, and formation of deacetyllanatoside C. The digoxin and purpureaglycoside A are intermediates of deacetyllanatoside C formation from digitoxin. Interactions between extracellular and intracellular compounds were considered. The proposed model could accurately predict cell growth, substrate consumption and product synthesis. And it can provide a useful framework for quantitative analysis of biotransformation in a plant cell culture system.
|
|
|
KEYWORD
|
|
|
kinetic model, Digitalis lanata, biotransformation, digitoxin, digoxin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|